Questions, methods and evidence

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Start with your biological question and inputs. Inspect the evaluations that inform it, the exact configurations tested and the evidence still missing.

2 matching use cases.

Proteins and complexes · Research / Clinical research

Assess methods for protein stability experiments

What evidence supports ranking protein substitutions by folding stability, and what must be validated before choosing a method?

Inputs: Wild-type protein sequence and amino acid substitutions; A defined construct and experimental stability endpoint; alignment-derived methods additionally require a traceable alignment and model

The current evidence is limited to the 47-residue AMFR_HUMAN_Tsuboyama_2023_4G3O construct in ProteinGym v1.3. Its cDNA-display proteolysis assay infers folding stability. Completed evaluations cover 2,972 variants: 820 single and 2,152 double substitutions.

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DNA and genomes · Research / Clinical research

Prioritise variants for splicing experiments

Which evaluated configurations can inform selection of human SNVs for follow-up splicing experiments?

Inputs: Human single nucleotide variants with alleles, genome assembly and gene/transcript context; The intended experimental endpoint and the number of variants that can be followed up

MFASS measures exon recognition in an artificial minigene reporter. The matched study evaluates genomic-context SpliceAI and Pangolin configurations against this functional endpoint on a fixed held-out population.

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About this collection

Use cases connect a research decision to existing tasks, evaluation protocols and source-linked results. They do not combine incompatible protocols into one ranking. Review method and missing evidence remain visible on every page.

Contribute evidence or a correction · Evidence and review methods

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Release 2026-09-25-8af07e960e5f

Use-case input digest d0c76e33f58fe8d4845cdba114d6bb6bea1920fc68c5a5e43922801c7e7ca27b

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