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NABAS+

NABAS+ performs species-level profiling of Illumina shotgun reads through reference alignment and stringent filtering.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Introduction (paragraph 3); Materials and methods/Statistical comparison and visualization (paragraph 1)

1 evaluation · 1 metric row

How it worksEvaluated procedure (conceptual)
Evaluated procedure (conceptual)1. Illumina shotgun metagenomic reads and a curated species reference database. Then: 2. NABAS+. Then: 3. Species identifications and microbiome profilesEvaluated procedure (conceptual)1. Illumina shotgun metagenomic reads and a curated species reference database. Then: 2. NABAS+. Then: 3. Species identifications and microbiome profilesEvaluated procedure (conceptual)1. Illumina shotgun metagenomic reads and a curated species reference database. Then: 2. NABAS+. Then: 3. Species identifications and microbiome profiles

Conceptual input–method–output guide. Check the procedure text and linked evaluation for fitted components, additional inputs and exact settings.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Running the classifiers (paragraph 2)

At a glance

limited source coverage · Automated source review, 2026-09-16. All specifications and missing details

Evaluations and results

Release 2026-09-17-d277315f7d76 · 1 evaluation · 1 metric row. Different protocols are not a single leaderboard.

Results grouped by the exact reported evaluation
Metric and findingCoverage and uncertaintyEvidence
NABAS+: Metagenomic taxonomic classification

Newly generated sample19 used for classifier comparison.

Author-reported evaluation · Evaluation metadata: needs review

0.719 F1 score

Unit: unitless · Direction: unknown

Uncertainty: not reported in legacy extract

Scored: Not reported · Eligible: Not reported

source checkedAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Table 3, Sample19-new / NABAS+ row, F1 score column

Source checking is not independent reproduction.

How it works

How the evaluated method works

BWA aligns reads to a curated RefSeq database with one selected genome per species. Quality filters retain genomes supported by reliable mapped reads.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Running the classifiers (paragraph 2)
What was evaluated

The linked evaluation record identifies NABAS+: Metagenomic taxonomic classification. Its dataset, split, adaptation and evidence origin remain attached to the reported results.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · The named evaluation’s methods and comparison table; exact preserved evaluation IDs: evaluation-lit-039

Strengths and limitations

Limitations and conditions

Profile review details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Stable record: reported-model-e7d203bd99ca99

Specifications

Inputs, training, access and other details

Explanatory profile: limited source coverage · Automated source review, 2026-09-16. Review applies to the cited claims; unresolved fields are listed below. Numerical results retain their own review status.

Inputs, outputs and configuration
PropertyDescription and evidence
Model typeStudy-specific predictive method; this record is the paper-specific evaluated configuration.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Running the classifiers (paragraph 2)
Architecture / procedureBWA aligns reads to a curated RefSeq database with one selected genome per species. Quality filters retain genomes supported by reliable mapped reads.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Running the classifiers (paragraph 2)
Biological inputsIllumina shotgun metagenomic reads and a curated species reference database
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Introduction (paragraph 3); Discussion (paragraph 6)
OutputsSpecies identifications and microbiome profiles
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Statistical comparison and visualization (paragraph 7); Discussion (paragraph 5)
ParametersAn aggregate parameter total for this exact evaluated configuration is not established by the inspected sources. · Not reported in inspected sources
Sources (2)Advancing metagenomic classification with NABAS+: a novel alignment-based approach; TakacsBertalan/NABAS_paper_scripts README.md · Materials and methods/Selecting and setting up classifiers for the initial gut metagenome analysis; Materials and methods/Selecting and setting up reference classifiers for NABAS+ benchmarking; Materials and methods/Datasets and reference databases/Human gut microbiome samples: acquisition and sequencing; Materials and methods/Datasets and reference databases/Consent for sample collection; Materials and methods/Datasets and reference databases/Real-world clinical dataset; Materials and methods/Datasets and reference databases/CAMI samples; Materials and methods/Datasets and reference databases/Recreating the CAMI sample19; Materials and methods/Datasets and reference databases/Zymo community standards; inspected for aggregate parameter count (component sizes are not added without an exact configuration); README.md at pinned repository revision
Known versions / configurationNABAS+ is the comparison-table label; that label does not specify an immutable weight revision. · Not reported in inspected sources
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Model identification in the comparison table and corresponding Methods; immutable checkpoint revision is not supplied by the table label.
Training data / fittingReference-genome curation rather than learned neural weights.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/Recreating the CAMI sample19 (paragraph 1); Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3)
Context limitsNot applicable to a pretrained sequence-token window; read/contig lengths and the reference-database or comparison configuration determine the analysed input. · Not applicable
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Discussion (paragraph 6)
AccessOfficial study implementation and usage documentation: https://github.com/TakacsBertalan/NABAS_paper_scripts/blob/7cab4d317a2c362988e7b96fb33f92a9c79a9fdc/README.md. This pinned documentation revision is not automatically the evaluated weight revision.
SourcesTakacsBertalan/NABAS_paper_scripts README.md · README.md; installation, model download and usage instructions
Code licenceCC BY-NC 4.0 (study repository code at the cited revision; this does not establish every dependency or historical checkpoint licence).
SourcesTakacsBertalan/NABAS_paper_scripts LICENSE · LICENSE; complete licence text
Weights licenceThe inspected model-access documentation does not explicitly identify terms for this exact evaluated checkpoint or fitted head; repository code terms are shown separately. · Not reported in inspected sources
SourcesTakacsBertalan/NABAS_paper_scripts README.md · README.md; checkpoint/access documentation and licence scope

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

20 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-09-17-d277315f7d76
Property and statementOriginal source and locationReview and provenance
Diagram caption

Conceptual input–method–output guide. Check the procedure text and linked evaluation for fitted components, additional inputs and exact settings.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Running the classifiers (paragraph 2)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.diagram.caption

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Diagram steps

["Illumina shotgun metagenomic reads and a curated species reference database","NABAS+","Species identifications and microbiome profiles"]

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Running the classifiers (paragraph 2)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.diagram.steps

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Diagram title

Evaluated procedure (conceptual)

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Running the classifiers (paragraph 2)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.diagram.title

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Model type

Study-specific predictive method; this record is the paper-specific evaluated configuration.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Running the classifiers (paragraph 2)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.0.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Architecture / procedure

BWA aligns reads to a curated RefSeq database with one selected genome per species. Quality filters retain genomes supported by reliable mapped reads.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Running the classifiers (paragraph 2)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.1.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Weights licence

The inspected model-access documentation does not explicitly identify terms for this exact evaluated checkpoint or fitted head; repository code terms are shown separately.

Individual claims
TakacsBertalan/NABAS_paper_scripts README.md

Original source ↗

README.md; checkpoint/access documentation and licence scope

Version: 7cab4d317a2c362988e7b96fb33f92a9c79a9fdc
Retrieved: 2026-09-16T19:54:19.540591+00:00

unreported

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.10.value

Source artifact SHA-256: 4dd306069e898af754cdf810adbcf7542a24918563d610933f50f48d41aeecac

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Biological inputs

Illumina shotgun metagenomic reads and a curated species reference database

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Introduction (paragraph 3); Discussion (paragraph 6)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.2.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Outputs

Species identifications and microbiome profiles

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Statistical comparison and visualization (paragraph 7); Discussion (paragraph 5)

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.3.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Parameters

An aggregate parameter total for this exact evaluated configuration is not established by the inspected sources.

Individual claims
TakacsBertalan/NABAS_paper_scripts README.md

Original source ↗

Materials and methods/Selecting and setting up classifiers for the initial gut metagenome analysis; Materials and methods/Selecting and setting up reference classifiers for NABAS+ benchmarking; Materials and methods/Datasets and reference databases/Human gut microbiome samples: acquisition and sequencing; Materials and methods/Datasets and reference databases/Consent for sample collection; Materials and methods/Datasets and reference databases/Real-world clinical dataset; Materials and methods/Datasets and reference databases/CAMI samples; Materials and methods/Datasets and reference databases/Recreating the CAMI sample19; Materials and methods/Datasets and reference databases/Zymo community standards; inspected for aggregate parameter count (component sizes are not added without an exact configuration); README.md at pinned repository revision

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: 7cab4d317a2c362988e7b96fb33f92a9c79a9fdc
Retrieved: 2026-09-16T19:54:19.540591+00:00

unreported

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.4.value

Source artifact SHA-256: 4dd306069e898af754cdf810adbcf7542a24918563d610933f50f48d41aeecac

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Parameters

An aggregate parameter total for this exact evaluated configuration is not established by the inspected sources.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Materials and methods/Selecting and setting up classifiers for the initial gut metagenome analysis; Materials and methods/Selecting and setting up reference classifiers for NABAS+ benchmarking; Materials and methods/Datasets and reference databases/Human gut microbiome samples: acquisition and sequencing; Materials and methods/Datasets and reference databases/Consent for sample collection; Materials and methods/Datasets and reference databases/Real-world clinical dataset; Materials and methods/Datasets and reference databases/CAMI samples; Materials and methods/Datasets and reference databases/Recreating the CAMI sample19; Materials and methods/Datasets and reference databases/Zymo community standards; inspected for aggregate parameter count (component sizes are not added without an exact configuration); README.md at pinned repository revision

Shared locator for this statement’s cited sources; not a separate locator for each citation.

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

unreported

automated source review · 2026-09-16

Audit details

Primary full text and the available official implementation/model documentation were inspected. Explanatory claims are source-backed; unresolved exact-configuration metadata is labelled explicitly. This is automated review, not a human review or independent benchmark reproduction.

Field: attributes.profile.facts.4.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Sources and history

Release 2026-09-17-d277315f7d76 · Record review: needs review

3 source records and release historyDownload this release
Technical metadata and extraction receipts

Stable ID: reported-model-e7d203bd99ca99

areas
microbes-communities
entity level
method
version
Not reported
reported name
NABAS+
historical missing metadata
version: not_reported_in_legacy_extract; checkpoint revision: not_reported_in_legacy_extract; training data: not_reported_in_legacy_extract; licence: not_reported_in_legacy_extract
metadata review scope
historical_missing_metadata preserves the original discovery state. Current descriptive evidence and missingness are recorded in profile.facts; numerical-result review is separate.
legacy kinds
model
entity classification
review date: 2026-09-17; rationale: This source-scoped entry preserves the method/configuration actually named in an evaluation. It is neither a global family identity nor proof of an immutable checkpoint; the linked evaluation retains adaptation, fitting and scoring details.; source ids: nabas-plus-2025; source locator: Materials and methods/Datasets and reference databases/CAMI samples (paragraph 3); Materials and methods/Running the classifiers (paragraph 2) | Introduction (paragraph 3); Materials and methods/Statistical comparison and visualization (paragraph 1); ambiguities: Configuration means the source-labelled evaluated identity. It does not establish missing checkpoint hashes, default settings or equivalence to same-named records in other papers.
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