rewire.it
Task

Metagenomic taxonomic classification

Metagenomic classification compares reference-based tools and examines dependence on reference-genome quality.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

2 evaluations · 2 metric rows

At a glance

Inputs, training, access and other details

Explanatory profile: limited source coverage · Automated source review, 2026-09-16. Review applies to the cited claims; unresolved fields are listed below. Numerical results retain their own review status.

Data, procedure and scoring
PropertyDescription and evidence
DatasetsCAMI2 Toy Human Microbiome Project samples are among the benchmark collections.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table
SplitsThis is evaluation of reference-database classifiers on CAMI2 samples and Zymo mock communities, rather than a supervised train/validation/test split. Reference-database versions are specified separately; the regenerated CAMI sample19 is a distinct condition. · Not applicable
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: CAMI2 reference databases, Recreating CAMI sample19, Zymo community standards, Running classifiers
MetricsPrecision, recall, F1, Jaccard distance and Bray–Curtis distance distinguish detection from abundance agreement.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table
BaselinesMetaPhlAn3, Kraken and GOTTCHA, using defaults where applicable.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table
Leakage controlsThe source discusses reference assembly age and database coverage as factors affecting apparent accuracy.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table
UncertaintyThe cited text-accessible evaluation sections give no confidence-interval, resampling or repeat-run error-bar specification. Image-only tables and uninspected supplements are outside this absence claim. · Not reported in inspected sources
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table
Entity typePaper-specific computational evaluation protocol.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table
OrganismsCAMI2 human-microbiome community taxa.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table
AssaysMetagenomic sequence mixtures with reference taxonomy.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table
Allowed inputsSequencing reads and model-specific reference databases.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table
AdaptationTaxonomic classification with default comparator settings where applicable.
SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

How it works

How it worksComputational evaluation flow
Computational evaluation flow1. Input: Sequencing reads and model-specific reference databases.. Then: 2. Evaluation: Taxonomic classification with default comparator settings where applicable.. Then: 3. Readout: Precision, recall, F1, Jaccard distance and Bray–Curtis distance distinguish detection from abundance agreement.Computational evaluation flow1. Input: Sequencing reads and model-specific reference databases.. Then: 2. Evaluation: Taxonomic classification with default comparator settings where applicable.. Then: 3. Readout: Precision, recall, F1, Jaccard distance and Bray–Curtis distance distinguish detection from abundance agreement.Computational evaluation flow1. Input: Sequencing reads and model-specific reference databases.. Then: 2. Evaluation: Taxonomic classification with default comparator settings where applicable.. Then: 3. Readout: Precision, recall, F1, Jaccard distance and Bray–Curtis distance distinguish detection from abundance agreement.

Conceptual summary of the cited evaluation; exact task configuration and source version remain part of the protocol.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table
Evaluation methodology

CAMI2 Toy Human Microbiome Project samples are among the benchmark collections. Precision, recall, F1, Jaccard distance and Bray–Curtis distance distinguish detection from abundance agreement. MetaPhlAn3, Kraken and GOTTCHA, using defaults where applicable. The source discusses reference assembly age and database coverage as factors affecting apparent accuracy. The cited text-accessible evaluation sections give no confidence-interval, resampling or repeat-run error-bar specification. Image-only tables and uninspected supplements are outside this absence claim.

SourcesAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

Recorded evaluations

Each evaluation records what was tested and under which conditions.

Tested entities and results

Release 2026-09-17-d277315f7d76 · 2 evaluations · 2 metric rows. Different protocols are not a single leaderboard.

Results grouped by the exact reported evaluation
Metric and findingCoverage and uncertaintyEvidence
NABAS+: Metagenomic taxonomic classification

Newly generated sample19 used for classifier comparison.

Author-reported evaluation · Evaluation metadata: needs review

0.719 F1 score

Unit: unitless · Direction: unknown

Uncertainty: not reported in legacy extract

Scored: Not reported · Eligible: Not reported

source checkedAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Table 3, Sample19-new / NABAS+ row, F1 score column

Source checking is not independent reproduction.

MetaPhlAn3: Metagenomic taxonomic classification

Newly generated sample19 used for classifier comparison.

Independent external evaluation · Evaluation metadata: needs review

0.753 F1 score

Unit: unitless · Direction: unknown

Uncertainty: not reported in legacy extract

Scored: Not reported · Eligible: Not reported

source checkedAdvancing metagenomic classification with NABAS+: a novel alignment-based approach · Table 3, Sample19-new / MetaPhlAn3 row, F1 score column

Source checking is not independent reproduction.

Papers and result coverage

Last literature check: 2026-09-17. Primary-source discovery and table/protocol screening; source checked is not independently reproduced. Raw acquisitions not automatically numerical publication approval.

Paper or primary resourceVersionReference
Advancing metagenomic classification with NABAS+: a novel alignment-based approachPMC12231603.1Read source
DOI: 10.1093/nargab/lqaf092

What is still missing

  • Complete raw tables acquired.19 new versusold reference experiments separate; Bray-Curtis lower-is-better unlike F1. Reference database state required before chart groups. Structured extraction pending.
Search and extraction details

source found structured extraction pending

Searches

  • Advancing metagenomic classification with NABAS+: a novel alignment-based approach primary paper benchmark results

Evidence locations

  • Tables3–4; new/old reference database and community datasets

Strengths and limitations

Strengths and considerations

No source-reviewed explanatory claims are recorded here yet.

Limitations and conditions

Profile review details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Stable record: reported-task-92137759a9e7b0

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

17 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-09-17-d277315f7d76
Property and statementOriginal source and locationReview and provenance
Diagram caption

Conceptual summary of the cited evaluation; exact task configuration and source version remain part of the protocol.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Field: attributes.profile.diagram.caption

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Diagram steps

["Input: Sequencing reads and model-specific reference databases.","Evaluation: Taxonomic classification with default comparator settings where applicable.","Readout: Precision, recall, F1, Jaccard distance and Bray–Curtis distance distinguish detection from abundance agreement."]

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Field: attributes.profile.diagram.steps

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Diagram title

Computational evaluation flow

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Field: attributes.profile.diagram.title

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Datasets

CAMI2 Toy Human Microbiome Project samples are among the benchmark collections.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Field: attributes.profile.facts.0.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Splits

This is evaluation of reference-database classifiers on CAMI2 samples and Zymo mock communities, rather than a supervised train/validation/test split. Reference-database versions are specified separately; the regenerated CAMI sample19 is a distinct condition.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Methods: CAMI2 reference databases, Recreating CAMI sample19, Zymo community standards, Running classifiers

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

inapplicable

automated source review · 2026-09-16

Audit details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Field: attributes.profile.facts.1.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Adaptation

Taxonomic classification with default comparator settings where applicable.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Field: attributes.profile.facts.10.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Metrics

Precision, recall, F1, Jaccard distance and Bray–Curtis distance distinguish detection from abundance agreement.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Field: attributes.profile.facts.2.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Baselines

MetaPhlAn3, Kraken and GOTTCHA, using defaults where applicable.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Field: attributes.profile.facts.3.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Leakage controls

The source discusses reference assembly age and database coverage as factors affecting apparent accuracy.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

source checked

automated source review · 2026-09-16

Audit details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Field: attributes.profile.facts.4.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Uncertainty

The cited text-accessible evaluation sections give no confidence-interval, resampling or repeat-run error-bar specification. Image-only tables and uninspected supplements are outside this absence claim.

Individual claims
Advancing metagenomic classification with NABAS+: a novel alignment-based approach

Original source ↗

Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table

Version: PMC12231603.1
Retrieved: 2026-09-16T10:41:16.546107+00:00

unreported

automated source review · 2026-09-16

Audit details

Relevant full-paper computational evaluation sections, tables/captions and cited supplementary task passages were reviewed. Reporting omissions are scoped to the inspected sources. Original numerical results are unchanged.

Field: attributes.profile.facts.5.value

Source artifact SHA-256: 49903e751beb86f6744825d2fdb3ea2fbe327b52b1ce68c323bfa8b66dae71ec

Hash scope: Hash scope not separately documented; inspect source record

Inspected artifact

Sources and history

Release 2026-09-17-d277315f7d76 · Record review: needs review

2 source records and release historyDownload this release
Technical metadata and extraction receipts

Stable ID: reported-task-92137759a9e7b0

areas
microbes-communities
tasks
Metagenomic taxonomic classification
entity level
task
version
Not reported
task
Metagenomic taxonomic classification
scope note
Paper-specific evaluation task; protocol completeness requires further extraction.
benchmark research
review date: 2026-09-17; status: source_found_structured_extraction_pending; primary sources: evidence-expansion-p2-nabas-plus-2025-49903e751beb; inspected locators: Tables3–4; new/old reference database and community datasets; searched queries: Advancing metagenomic classification with NABAS+: a novel alignment-based approach primary paper benchmark results; gaps: Complete raw tables acquired.19 new versusold reference experiments separate; Bray-Curtis lower-is-better unlike F1. Reference database state required before chart groups. Structured extraction pending.; claim scope: Primary-source discovery and table/protocol screening; source checked is not independently reproduced. Raw acquisitions not automatically numerical publication approval.
historical missing metadata
protocol version: not_reported_in_legacy_extract; split: not_reported_in_legacy_extract
metadata review scope
historical_missing_metadata preserves the original discovery state. Current descriptive evidence and missingness are recorded in profile.facts; numerical-result review is separate.
legacy kinds
benchmark
entity classification
review date: 2026-09-17; rationale: This source-scoped record identifies the biological prediction task and holds its paper context. Preserve the existing task identity; exact split, model adaptation and scoring remain in linked evaluations or separate protocol records.; source ids: nabas-plus-2025; source locator: Methods: reference classifiers; Discussion: CAMI evaluation; cached text lines 13–15, 108–109; task metric definitions and corresponding results table; ambiguities: A paper- or suite-specific task may constrain some inputs or metrics; that alone does not make it interchangeable with a complete versioned protocol. No protocol equivalence is inferred.; Some legacy profile Entity type facts use the generic phrase computational evaluation protocol. That boilerplate is not sufficient to establish a single fixed protocol identity or to merge this task with another protocol record.
Related records

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