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Seurat reference mapping (SCTransform)

Seurat reference mapping is a cell type label transfer method provided by the Seurat package. Gene expression counts are first normalised by SCTransform before computing PCA. Then it finds mutual nearest neighbours, known as transfer anchors, between the labelled and unlabelled part of the data in PCA space, and computes each cell’s distance to each of the anchor pairs. Finally, it uses the labelled anchors to predict cell types for unlabelled cells based on these distances.

24 evaluations · 24 metric rows

Overview

Seurat reference mapping is a cell type label transfer method provided by the Seurat package. Gene expression counts are first normalised by SCTransform before computing PCA. Then it finds mutual nearest neighbours, known as transfer anchors, between the labelled and unlabelled part of the data in PCA space, and computes each cell’s distance to each of the anchor pairs. Finally, it uses the labelled anchors to predict cell types for unlabelled cells based on these distances.

Consult the linked sources for architecture or protocol details. Missing evidence is not evidence of a missing capability.

Evaluations and results

Release 2026-09-17-134cd1815de8 · 24 evaluations · 24 metric rows. Different protocols are not a single leaderboard. Where several source tables report the same metric, the published comparisons above offer a pooled view that names what it does not hold constant.

Results grouped by the exact reported evaluation
Metric and findingCoverage and uncertaintyEvidence
Seurat reference mapping (SCTransform) on Open Problems label projection CENGEN-BATCH-ACCURACY: Label projection on CeNGEN (split by batch), Accuracy

100k FACS-isolated C. elegans neurons from 17 experiments sequenced on 10x Genomics. Split into train/test by experimental batch. Dimensions: 100955 cells, 22469 genes. 169 cell types (avg. 597±800 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.8478130617136009 accuracy

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(cengen_batch), method(seurat_reference_mapping), paramset(SCTransform), metric(accuracy)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection CENGEN-BATCH-F1: Label projection on CeNGEN (split by batch), F1 score

100k FACS-isolated C. elegans neurons from 17 experiments sequenced on 10x Genomics. Split into train/test by experimental batch. Dimensions: 100955 cells, 22469 genes. 169 cell types (avg. 597±800 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.8432431418068973 f1

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(cengen_batch), method(seurat_reference_mapping), paramset(SCTransform), metric(f1)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection CENGEN-BATCH-F1-MACRO: Label projection on CeNGEN (split by batch), Macro F1 score

100k FACS-isolated C. elegans neurons from 17 experiments sequenced on 10x Genomics. Split into train/test by experimental batch. Dimensions: 100955 cells, 22469 genes. 169 cell types (avg. 597±800 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.4796671443590633 f1-macro

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(cengen_batch), method(seurat_reference_mapping), paramset(SCTransform), metric(f1_macro)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection CENGEN-RANDOM-ACCURACY: Label projection on CeNGEN (random split), Accuracy

100k FACS-isolated C. elegans neurons from 17 experiments sequenced on 10x Genomics. Split into train/test randomly. Dimensions: 100955 cells, 22469 genes. 169 cell types avg. 597±800 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.8321474453997431 accuracy

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(cengen_random), method(seurat_reference_mapping), paramset(SCTransform), metric(accuracy)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection CENGEN-RANDOM-F1: Label projection on CeNGEN (random split), F1 score

100k FACS-isolated C. elegans neurons from 17 experiments sequenced on 10x Genomics. Split into train/test randomly. Dimensions: 100955 cells, 22469 genes. 169 cell types avg. 597±800 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.830794751253811 f1

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(cengen_random), method(seurat_reference_mapping), paramset(SCTransform), metric(f1)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection CENGEN-RANDOM-F1-MACRO: Label projection on CeNGEN (random split), Macro F1 score

100k FACS-isolated C. elegans neurons from 17 experiments sequenced on 10x Genomics. Split into train/test randomly. Dimensions: 100955 cells, 22469 genes. 169 cell types avg. 597±800 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.7342634929447799 f1-macro

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(cengen_random), method(seurat_reference_mapping), paramset(SCTransform), metric(f1_macro)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection PANCREAS-BATCH-ACCURACY: Label projection on Pancreas (by batch), Accuracy

Human pancreatic islet scRNA-seq data from 6 datasets across technologies (CEL-seq, CEL-seq2, Smart-seq2, inDrop, Fluidigm C1, and SMARTER-seq). Split into train/test by experimental batch. Dimensions: 16382 cells, 18771 genes. 14 cell types (avg. 1170±1703 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.9588855829050582 accuracy

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(pancreas_batch), method(seurat_reference_mapping), paramset(SCTransform), metric(accuracy)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection PANCREAS-BATCH-F1: Label projection on Pancreas (by batch), F1 score

Human pancreatic islet scRNA-seq data from 6 datasets across technologies (CEL-seq, CEL-seq2, Smart-seq2, inDrop, Fluidigm C1, and SMARTER-seq). Split into train/test by experimental batch. Dimensions: 16382 cells, 18771 genes. 14 cell types (avg. 1170±1703 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.9582796607282135 f1

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(pancreas_batch), method(seurat_reference_mapping), paramset(SCTransform), metric(f1)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection PANCREAS-BATCH-F1-MACRO: Label projection on Pancreas (by batch), Macro F1 score

Human pancreatic islet scRNA-seq data from 6 datasets across technologies (CEL-seq, CEL-seq2, Smart-seq2, inDrop, Fluidigm C1, and SMARTER-seq). Split into train/test by experimental batch. Dimensions: 16382 cells, 18771 genes. 14 cell types (avg. 1170±1703 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.8517420844591291 f1-macro

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(pancreas_batch), method(seurat_reference_mapping), paramset(SCTransform), metric(f1_macro)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection PANCREAS-RANDOM-ACCURACY: Label projection on Pancreas (random split), Accuracy

Human pancreatic islet scRNA-seq data from 6 datasets across technologies (CEL-seq, CEL-seq2, Smart-seq2, inDrop, Fluidigm C1, and SMARTER-seq). Split into train/test randomly. Dimensions: 16382 cells, 18771 genes. 14 cell types (avg. 1170±1703 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.982370820668693 accuracy

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(pancreas_random), method(seurat_reference_mapping), paramset(SCTransform), metric(accuracy)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection PANCREAS-RANDOM-F1: Label projection on Pancreas (random split), F1 score

Human pancreatic islet scRNA-seq data from 6 datasets across technologies (CEL-seq, CEL-seq2, Smart-seq2, inDrop, Fluidigm C1, and SMARTER-seq). Split into train/test randomly. Dimensions: 16382 cells, 18771 genes. 14 cell types (avg. 1170±1703 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.9816543811179429 f1

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(pancreas_random), method(seurat_reference_mapping), paramset(SCTransform), metric(f1)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection PANCREAS-RANDOM-F1-MACRO: Label projection on Pancreas (random split), Macro F1 score

Human pancreatic islet scRNA-seq data from 6 datasets across technologies (CEL-seq, CEL-seq2, Smart-seq2, inDrop, Fluidigm C1, and SMARTER-seq). Split into train/test randomly. Dimensions: 16382 cells, 18771 genes. 14 cell types (avg. 1170±1703 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.8948274458832942 f1-macro

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(pancreas_random), method(seurat_reference_mapping), paramset(SCTransform), metric(f1_macro)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection PANCREAS-RANDOM-LABEL-NOISE-ACCURACY: Label projection on Pancreas (random split with label noise), Accuracy

Human pancreatic islet scRNA-seq data from 6 datasets across technologies (CEL-seq, CEL-seq2, Smart-seq2, inDrop, Fluidigm C1, and SMARTER-seq). Split into train/test randomly with 20% label noise. Dimensions: 16382 cells, 18771 genes. 14 cell types (avg. 1170±1703 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.9756548536209553 accuracy

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(pancreas_random_label_noise), method(seurat_reference_mapping), paramset(SCTransform), metric(accuracy)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection PANCREAS-RANDOM-LABEL-NOISE-F1: Label projection on Pancreas (random split with label noise), F1 score

Human pancreatic islet scRNA-seq data from 6 datasets across technologies (CEL-seq, CEL-seq2, Smart-seq2, inDrop, Fluidigm C1, and SMARTER-seq). Split into train/test randomly with 20% label noise. Dimensions: 16382 cells, 18771 genes. 14 cell types (avg. 1170±1703 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.975085587274387 f1

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(pancreas_random_label_noise), method(seurat_reference_mapping), paramset(SCTransform), metric(f1)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection PANCREAS-RANDOM-LABEL-NOISE-F1-MACRO: Label projection on Pancreas (random split with label noise), Macro F1 score

Human pancreatic islet scRNA-seq data from 6 datasets across technologies (CEL-seq, CEL-seq2, Smart-seq2, inDrop, Fluidigm C1, and SMARTER-seq). Split into train/test randomly with 20% label noise. Dimensions: 16382 cells, 18771 genes. 14 cell types (avg. 1170±1703 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.8955432565937734 f1-macro

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(pancreas_random_label_noise), method(seurat_reference_mapping), paramset(SCTransform), metric(f1_macro)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection TABULA-MURIS-SENIS-LUNG-RANDOM-ACCURACY: Label projection on Tabula Muris Senis Lung (random split), Accuracy

All lung cells from Tabula Muris Senis, a 500k cell-atlas from 18 organs and tissues across the mouse lifespan. Split into train/test randomly. Dimensions: 24540 cells, 17985 genes. 39 cell types (avg. 629±999 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.9119358074222668 accuracy

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(tabula_muris_senis_lung_random), method(seurat_reference_mapping), paramset(SCTransform), metric(accuracy)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection TABULA-MURIS-SENIS-LUNG-RANDOM-F1: Label projection on Tabula Muris Senis Lung (random split), F1 score

All lung cells from Tabula Muris Senis, a 500k cell-atlas from 18 organs and tissues across the mouse lifespan. Split into train/test randomly. Dimensions: 24540 cells, 17985 genes. 39 cell types (avg. 629±999 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.909781894158941 f1

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(tabula_muris_senis_lung_random), method(seurat_reference_mapping), paramset(SCTransform), metric(f1)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection TABULA-MURIS-SENIS-LUNG-RANDOM-F1-MACRO: Label projection on Tabula Muris Senis Lung (random split), Macro F1 score

All lung cells from Tabula Muris Senis, a 500k cell-atlas from 18 organs and tissues across the mouse lifespan. Split into train/test randomly. Dimensions: 24540 cells, 17985 genes. 39 cell types (avg. 629±999 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.8526389520351096 f1-macro

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(tabula_muris_senis_lung_random), method(seurat_reference_mapping), paramset(SCTransform), metric(f1_macro)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection ZEBRAFISH-LABS-ACCURACY: Label projection on Zebrafish (by laboratory), Accuracy

90k cells from zebrafish embryos throughout the first day of development, with and without a knockout of chordin, an important developmental gene. Split into train/test by laboratory. Dimensions: 26022 cells, 25258 genes. 24 cell types (avg. 1084±1156 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.3442973919813157 accuracy

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(zebrafish_labs), method(seurat_reference_mapping), paramset(SCTransform), metric(accuracy)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection ZEBRAFISH-LABS-F1: Label projection on Zebrafish (by laboratory), F1 score

90k cells from zebrafish embryos throughout the first day of development, with and without a knockout of chordin, an important developmental gene. Split into train/test by laboratory. Dimensions: 26022 cells, 25258 genes. 24 cell types (avg. 1084±1156 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.41030222359279184 f1

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(zebrafish_labs), method(seurat_reference_mapping), paramset(SCTransform), metric(f1)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection ZEBRAFISH-LABS-F1-MACRO: Label projection on Zebrafish (by laboratory), Macro F1 score

90k cells from zebrafish embryos throughout the first day of development, with and without a knockout of chordin, an important developmental gene. Split into train/test by laboratory. Dimensions: 26022 cells, 25258 genes. 24 cell types (avg. 1084±1156 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.28347167382711347 f1-macro

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(zebrafish_labs), method(seurat_reference_mapping), paramset(SCTransform), metric(f1_macro)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection ZEBRAFISH-RANDOM-ACCURACY: Label projection on Zebrafish (random split), Accuracy

90k cells from zebrafish embryos throughout the first day of development, with and without a knockout of chordin, an important developmental gene. Split into train/test randomly. Dimensions: 26022 cells, 25258 genes. 24 cell types (avg. 1084±1156 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.8596287703016241 accuracy

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(zebrafish_random), method(seurat_reference_mapping), paramset(SCTransform), metric(accuracy)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection ZEBRAFISH-RANDOM-F1: Label projection on Zebrafish (random split), F1 score

90k cells from zebrafish embryos throughout the first day of development, with and without a knockout of chordin, an important developmental gene. Split into train/test randomly. Dimensions: 26022 cells, 25258 genes. 24 cell types (avg. 1084±1156 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.8577182706160404 f1

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(zebrafish_random), method(seurat_reference_mapping), paramset(SCTransform), metric(f1)

Source checking is not independent reproduction.

Seurat reference mapping (SCTransform) on Open Problems label projection ZEBRAFISH-RANDOM-F1-MACRO: Label projection on Zebrafish (random split), Macro F1 score

90k cells from zebrafish embryos throughout the first day of development, with and without a knockout of chordin, an important developmental gene. Split into train/test randomly. Dimensions: 26022 cells, 25258 genes. 24 cell types (avg. 1084±1156 cells per cell type).

Author-reported evaluation · Evaluation metadata: source checked

0.7417937504863691 f1-macro

Unit: fraction · Direction: higher

Uncertainty: Not reported

Scored: Not reported · Eligible: Not reported

source checkedopenproblems-label primary benchmark evidence · results, dataset(zebrafish_random), method(seurat_reference_mapping), paramset(SCTransform), metric(f1_macro)

Source checking is not independent reproduction.

Evidence table

Inspect claims, sources and review details

Trace each statement to its source and review. A context-only reference supports the record generally; it does not verify an individual field. Source checking does not reproduce an experiment.

One row per statement and cited source. Multiple citations are not independent evaluations. Shared locators are labelled explicitly.

0 evidence rows matching the loaded filters

Claims, original sources and review scope · Release 2026-09-17-134cd1815de8
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Sources and history

Release 2026-09-17-134cd1815de8 · Record review: source checked

1 source records and release historyDownload this release
Technical metadata and extraction receipts

Stable ID: open-problems-method-seurat-reference-mapping-sctransform

areas
cells-tissues
source locator
results, method(seurat_reference_mapping), paramset(SCTransform)
missing metadata
checkpoint revision: unreported; parameters: unextracted
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